Serologic study on leptospiral infection in sheep in Ahvaz, southwestern Iran
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Abstract:
To investigate the seroprevalence of leptospiral infection in sheep in Ahvaz, southwestern Iran, bloodsamples were taken from 181 female sheep. Sera were stored at -20°C until use. They were initially screenedat serum dilution of 1:100 against six live antigens of Leptospira interrogans serovars pomona, canicola,hardjo, ballum, icterohaemorrhagiae, grippotyphosa using the microscopic agglutination test (MAT). Thesamples were considered positive if ≥ 50% of agglutination of leptospire in a dilution test serum of ≥ l:100were observed. Sera with positive results were titrated against reacting antigens in serial two-fold dilutionsfrom l:100 to 1:1600. Antibodies against one or more serovars were detected in 27 (14.9%) sera at dilution≥1:100. Antibodies against more than one serovar were found in 5 (18.5%) positive sera. Among the positivesera, antibodies were most frequent to serovar pomona (43.8%) followed by canicola (21.9%),icterohaemorrhagiae (12.5%), grippotyphosa (9.4%), ballum and hardjo (each of them 6.3%). The results ofthis survey indicate that leptospiral infection is common in sheep in Ahvaz and that various serovars concurin the etiology.
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Journal title
volume 8 issue 4
pages 333- 336
publication date 2007-12-01
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